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AJICAP: Affinity Peptide Mediated Regiodivergent Functionalization of Native Antibodies
Author(s) -
Yamada Kei,
Shikida Natsuki,
Shimbo Kazutaka,
Ito Yuji,
Khedri Zahra,
Matsuda Yutaka,
Mendelsohn Brian A.
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201814215
Subject(s) - peptide , biomolecule , surface plasmon resonance , chemistry , combinatorial chemistry , covalent bond , antibody , surface modification , lysine , conjugate , amino acid , peptide library , thiol , biochemistry , stereochemistry , peptide sequence , nanotechnology , biology , immunology , organic chemistry , materials science , nanoparticle , mathematical analysis , mathematics , gene
The need for atom‐precise biomolecule modification, and particularly the irreversible formation of covalent bonds to specific amino acids in proteins, has become an essential issue in the fields of pharmaceuticals and chemical biology. For example, antibody–drug conjugates (ADCs) are increasingly common entries into the clinical oncology pipeline. Herein, we report a new method of affinity peptide mediated regiodivergent functionalization (AJICAP™) that enables the synthesis of ADCs from native IgG antibodies. We succeeded in introducing thiol functional groups onto three lysine residues in IgGs using Fc affinity peptide reagents without antibody engineering. A cytotoxic molecule was then connected to the newly introduced thiol group, and both a surface plasmon resonance binding assay and in vivo xenograft mouse model results showed that the resulting ADC could selectively target and kill HER2‐positive cells. Our strategy provides a new approach for constructing complex antibody‐derived biomolecules.