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A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
Author(s) -
Sundukova Mayya,
Prifti Efthymia,
Bucci Annalisa,
Kirillova Kseniia,
Serrao Joana,
Reymond Luc,
Umebayashi Miwa,
Hovius Ruud,
Riezman Howard,
Johnsson Kai,
Heppenstall Paul A.
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201812967
Subject(s) - sensitivity (control systems) , fluorescence , voltage , neuroscience , membrane , interrogation , computer science , nanotechnology , chemistry , biological system , biology , materials science , physics , electronic engineering , electrical engineering , engineering , optics , biochemistry , geography , archaeology
Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons.