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Development of α‐Gal–Antibody Conjugates to Increase Immune Response by Recruiting Natural Antibodies
Author(s) -
Sianturi Julinton,
Manabe Yoshiyuki,
Li HaoSheng,
Chiu LiTing,
Chang TsungChe,
Tokunaga Kento,
Kabayama Kazuya,
Tanemura Masahiro,
Takamatsu Shinji,
Miyoshi Eiji,
Hung ShangCheng,
Fukase Koichi
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201812914
Subject(s) - antibody , conjugate , potency , cytotoxicity , chemistry , conjugated system , immune system , dendrimer , antigen , biochemistry , in vitro , immunology , biology , mathematical analysis , mathematics , organic chemistry , polymer
Cancer treatment with antibodies (Abs) is one of the most successful therapeutic strategies for obtaining high selectivity. In this study, α‐gal–Ab conjugates were developed that dramatically increased cellular cytotoxicity by recruiting natural Abs through the interaction between α‐gal and anti‐gal Abs. The potency of the α‐gal–Ab conjugates depended on the amount of α‐gal conjugated to the antibody: the larger the amount of α‐gal introduced, the higher the level of cytotoxicity observed. The conjugation of antibodies with an α‐gal dendrimer allowed the introduction of large amounts of α‐gal to the Ab, without loss of affinity for the target cell. The method described here will enable the re‐development of Abs to improve their potency.