Premium
Boron‐Containing Probes for Non‐optical High‐Resolution Imaging of Biological Samples
Author(s) -
Kabatas Selda,
AgüiGonzalez Paola,
Saal KimAnn,
Jähne Sebastian,
Opazo Felipe,
Rizzoli Silvio O.,
Phan Nhu T. N.
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201812032
Subject(s) - boron , mass spectrometry , chemistry , ion , nanotechnology , molecular imaging , biological imaging , biological materials , analytical chemistry (journal) , biophysics , materials science , fluorescence , biological system , biology , chromatography , physics , microbiology and biotechnology , organic chemistry , quantum mechanics , in vivo
Boron has been employed in materials science as a marker for imaging specific structures by electron energy loss spectroscopy (EELS) or secondary ion mass spectrometry (SIMS). It has a strong potential in biological analyses as well; however, the specific coupling of a sufficient number of boron atoms to a biological structure has proven challenging. Herein, we synthesize tags containing closo ‐1,2‐dicarbadodecaborane, coupled to soluble peptides, which were integrated in specific proteins by click chemistry in mammalian cells and were also coupled to nanobodies for use in immunocytochemistry experiments. The tags were fully functional in biological samples, as demonstrated by nanoSIMS imaging of cell cultures. The boron signal revealed the protein of interest, while other SIMS channels were used for imaging different positive ions, such as the cellular metal ions. This allows, for the first time, the simultaneous imaging of such ions with a protein of interest and will enable new biological applications in the SIMS field.