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A Pretreatment‐Free, Polymer‐Based Platform Prepared by Molecular Imprinting and Post‐Imprinting Modifications for Sensing Intact Exosomes
Author(s) -
Mori Kisho,
Hirase Mitsuhiro,
Morishige Takahiro,
Takano Eri,
Sunayama Hirobumi,
Kitayama Yukiya,
Inubushi Sachiko,
Sasaki Ryohei,
Yashiro Masakazu,
Takeuchi Toshifumi
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201811142
Subject(s) - microvesicles , imprinting (psychology) , molecular imprinting , fluorescence , ultracentrifuge , chemistry , exosome , biophysics , vesicle , membrane , nanotechnology , microbiology and biotechnology , biology , materials science , biochemistry , gene , microrna , physics , quantum mechanics , selectivity , catalysis
Abstract Exosomes are small (30–100 nm) membrane vesicles that serve as regulatory agents for intercellular communication in cancers. Currently, exosomes are detected by immuno‐based assays with appropriate pretreatments like ultracentrifugation and are time consuming (>12 h). We present a novel pretreatment‐free fluorescence‐based sensing platform for intact exosomes, wherein exchangeable antibodies and fluorescent reporter molecules were aligned inside exosome‐binding cavities. Such antibody‐containing fluorescent reporter‐grafted nanocavities were prepared on a substrate by well‐designed molecular imprinting and post‐imprinting modifications to introduce antibodies and fluorescent reporter molecules only inside the binding nanocavities, enabling sufficiently high sensitivity to detect intact exosomes without pretreatment. The effectiveness of the system was demonstrated by using it to discriminate between normal exosomes and those originating from prostate cancer and analyze exosomes in tear drops.