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A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging
Author(s) -
Sheng Wei,
Nick Setare Tahmasebi,
Santos Elizabeth M.,
Ding Xinliang,
Zhang Jun,
Vasileiou Chrysoula,
Geiger James H.,
Borhan Babak
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201810065
Subject(s) - fluorophore , chromophore , photoisomerization , bathochromic shift , fluorescence , chemistry , photochemistry , imine , fluorene , biophysics , protonation , isomerization , biochemistry , biology , organic chemistry , optics , catalysis , ion , physics , polymer
FR‐1V , a fluorene‐based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF‐2 , a prototypic protein–dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV‐light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no‐wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.