A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging | Zendy

Sheng Wei | Zendy; Nick Setare Tahmasebi | Zendy; Santos Elizabeth M. | Zendy; Ding Xinliang | Zendy; Zhang Jun | Zendy; Vasileiou Chrysoula | Zendy; Geiger James H. | Zendy; Borhan Babak | Zendy

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A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging

Author(s) -

Sheng Wei,

Nick Setare Tahmasebi,

Santos Elizabeth M.,

Ding Xinliang,

Zhang Jun,

Vasileiou Chrysoula,

Geiger James H.,

Borhan Babak

Publication year - 2018

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201810065

Subject(s) - fluorophore , chromophore , photoisomerization , chemistry , fluorescence , bathochromic shift , photochemistry , imine , fluorene , protein tag , protonation , biophysics , isomerization , biochemistry , organic chemistry , biology , catalysis , ion , physics , polymer , quantum mechanics , fusion protein , gene , recombinant dna

FR‐1V , a fluorene‐based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF‐2 , a prototypic protein–dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV‐light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no‐wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.

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