Premium
Transcript Profiling of MRSA Biofilms Treated with a Halogenated Phenazine Eradicating Agent: A Platform for Defining Cellular Targets and Pathways Critical to Biofilm Survival
Author(s) -
Abouelhassan Yasmeen,
Zhang Yanping,
Jin Shouguang,
Huigens Robert W.
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201809785
Subject(s) - biofilm , kegg , biology , microbiology and biotechnology , arginine deiminase , atp binding cassette transporter , multidrug tolerance , gene expression , proteases , transporter , gene expression profiling , bacteria , gene , transcriptome , biochemistry , enzyme , arginine , genetics , amino acid
Bacterial biofilms are surface‐attached communities of non‐replicating bacteria innately tolerant to antibiotics. Biofilms display differential gene expression profiles and physiologies as compared to their planktonic counterparts; however, their biology remains largely unknown. In this study, we used a halogenated phenazine (HP) biofilm eradicator in transcript profiling experiments (RNA‐seq) to define cellular targets and pathways critical to biofilm viability. WoPPER analysis with time–course validation (RT‐qPCR) revealed that HP‐14 induces rapid iron starvation in MRSA biofilms, as evident by the activation of iron‐acquisition gene clusters in 1 hour. Serine proteases and oligopeptide transporters were also found to be up‐regulated, whereas glycolysis, arginine deiminase, and urease gene clusters were down‐regulated. KEGG analysis revealed that HP‐14 impacts metabolic and ABC transporter functional pathways. These findings suggest that MRSA biofilm viability relies on iron homeostasis.