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Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase
Author(s) -
Sanzhaeva Urikhan,
Xu Xuan,
Guggilapu Priyaankadevi,
Tseytlin Mark,
Khramtsov Valery V.,
Driesschaert Benoit
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201806851
Subject(s) - electron paramagnetic resonance , alkaline phosphatase , substrate (aquarium) , enzyme , paramagnetism , enzyme assay , in vivo , chemistry , selectivity , nuclear magnetic resonance , magnetic resonance imaging , phosphatase , materials science , biochemistry , biology , physics , medicine , catalysis , condensed matter physics , ecology , microbiology and biotechnology , radiology
Enzyme activities are well established biomarkers of many pathologies. Imaging enzyme activity directly in vivo may help to gain insight into the pathogenesis of various diseases but remains extremely challenging. In this communication, we report the use of EPR imaging (EPRI) in combination with a specially designed paramagnetic enzymatic substrate to map alkaline phosphatase activity with a high selectivity, thereby demonstrating the potential of EPRI to map enzyme activity.