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DNA‐Mediated Proximity‐Based Assembly Circuit for Actuation of Biochemical Reactions
Author(s) -
Oh Sung Won,
Pereira Adriana,
Zhang Ting,
Li Tianran,
Lane Ariel,
Fu Jinglin
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201806749
Subject(s) - dna , cofactor , aptamer , nanotechnology , biosensor , chemistry , biophysics , molecular switch , dna nanotechnology , combinatorial chemistry , computational biology , enzyme , molecule , materials science , biochemistry , biology , microbiology and biotechnology , organic chemistry
Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point‐of‐care (POC) diagnostic applications. Herein, the development of a DNA‐mediated proximity assembly of biochemical reactions, which was capable of sensing various bio‐targets and reporting easy‐to‐read signals is reported. The circuit was composed of a DNA hairpin‐locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests.