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Self‐Assembled Functional DNA Superstructures as High‐Density and Versatile Recognition Elements for Printed Paper Sensors
Author(s) -
Liu Meng,
Zhang Qiang,
Kannan Balamurali,
Botton Gianluigi A.,
Yang Jie,
Soleymani Leyla,
Brennan John D.,
Li Yingfu
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201806489
Subject(s) - dna , deoxyribozyme , physisorption , aptamer , biosensor , nanotechnology , chemistry , nucleic acid , self assembly , rolling circle replication , combinatorial chemistry , adsorption , materials science , biochemistry , organic chemistry , polymerase , microbiology and biotechnology , biology
Micrometer‐sized functional nucleic acid (FNA) superstructures (denoted as 3D DNA) were examined as a unique class of biorecognition elements to produce highly functional bioactive paper surfaces. 3D DNA containing repeating sequences of either a DNA aptamer or DNAzyme was created from long‐chain products of rolling circle amplification followed by salt aging. The resulting 3D DNA retained its original spherical shape upon inkjet printing and adhered strongly to the paper surface via physisorption. 3D DNA paper sensors showed resistance to degradation by nucleases, suppressed nonspecific protein adsorption, and provided a much higher surface density of functional DNA relative to monomeric FNAs, making such species ideally suited for development of paper‐based biosensors.