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Enzyme–Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One‐Pot Cascade Reactions
Author(s) -
Sun Zhiyong,
Glebe Ulrich,
Charan Himanshu,
Böker Alexander,
Wu Changzhu
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201806049
Subject(s) - pickering emulsion , conjugate , catalysis , emulsion , biocatalysis , polymer , benzaldehyde , chemistry , cascade , enzyme catalysis , immobilized enzyme , enzyme , chemical engineering , organic chemistry , materials science , cascade reaction , combinatorial chemistry , chromatography , reaction mechanism , engineering , mathematical analysis , mathematics
Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid–liquid interfaces with chemocatalysts, the use of unstable biocatalysts at emulsion interfaces remains a technical challenge. Herein, we present a Pickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme–polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions. The conjugates were prepared by growing poly( N ‐isopropylacrylamide) on a fragile enzyme, benzaldehyde lyase, under physiological conditions. The mild in situ conjugation process preserved the enzyme structure, and the conjugates were used to emulsify a water–organic two‐phase system into a stable Pickering emulsion, leading to a significantly larger interfacial area and a 270‐fold improvement in catalytic performance as compared to the unemulsified two‐phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.