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Abiotic Sequence‐Coded Oligomers as Efficient In Vivo Taggants for the Identification of Implanted Materials
Author(s) -
Karamessini Denise,
SimonYarza Teresa,
Poyer Salomé,
Konishcheva Evgeniia,
Charles Laurence,
Letourneur Didier,
Lutz JeanFrançois
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201804895
Subject(s) - in vivo , monomer , mass spectrometry , solvent , sequence (biology) , chemistry , materials science , implant , tandem mass spectrometry , nuclear chemistry , chromatography , polymer , organic chemistry , biochemistry , surgery , biology , medicine , microbiology and biotechnology
Sequence‐defined oligourethanes were tested as in vivo taggants for implant identification. The oligomers were prepared in an orthogonal solid‐phase iterative approach and thus contained a coded monomer sequence that can be unequivocally identified by tandem mass spectrometry (MS/MS). The oligomers were then included in small amounts (1 wt %) in square‐centimeter‐sized crosslinked poly(vinyl alcohol) (PVA) model films, which were intramuscularly and subcutaneously implanted in the abdomen of rats. After one week, one month, or three months of implantation, the PVA films were explanted. The rat tissues exposed to the implants did not exhibit any adverse reactions, which suggested that the taggants are not harmful and probably not leaching out from the films. Furthermore, the explanted films were immersed in methanol, as a solvent for oligourethanes, and the liquid extract was analyzed by mass spectrometry. In all cases, the oligourethane taggant was detected, and its sequence was identified by MS/MS.