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A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging
Author(s) -
Grzybowski Marek,
Taki Masayasu,
Senda Kieko,
Sato Yoshikatsu,
Ariyoshi Tetsuro,
Okada Yasushi,
Kawakami Ryosuke,
Imamura Takeshi,
Yamaguchi Shigehiro
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201804731
Subject(s) - fluorescence , rhodamine , rhodamine b , microscopy , fluorescence lifetime imaging microscopy , photochemistry , fluorescence microscope , biomolecule , chemistry , infrared , dextran , biophysics , materials science , nanotechnology , chromatography , optics , organic chemistry , biology , medicine , pathology , physics , photocatalysis , catalysis
Various fluorescence microscopy techniques require bright NIR‐emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus‐substituted rhodamine dyes (PORs) upon substitution at the 9‐position with a 2,6‐dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long‐term and three‐color imaging in the vis‐NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye‐labeled biomolecule by single‐molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR‐emitting PREX 710‐dextran conjugate.