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On the Development of Electron Cryo‐Microscopy (Nobel Lecture)
Author(s) -
Dubochet Jacques
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201804280
Subject(s) - electron microscope , cryo electron microscopy , nanotechnology , microscopy , biological specimen , materials science , chemistry , physics , optics , biochemistry
An electron microscope must operate under vacuum which means that any observed biological specimen must be dry. When water is removed, however, the molecules stick to each other. J. Dubochet describes in his Nobel lecture the solution to this dilemma that paved the way to electron‐cryo microscopy: suspension of the specimen in vitrifying water.