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Rapid Photoactuation of a DNA Nanostructure using an Internal Photocaged Trigger Strand
Author(s) -
Liu Minghui,
Jiang Shuoxing,
Loza Omar,
Fahmi Nour Eddine,
Šulc Petr,
Stephanopoulos Nicholas
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201804264
Subject(s) - dna , chemistry , intramolecular force , single strand , biophysics , nanotechnology , materials science , stereochemistry , biology , biochemistry
A reconfigurable DNA nano‐tweezer is reported that can be switched between a closed and open state with a brief pulse of UV light. In its initial state, the tweezer is held shut using a hairpin with a single‐stranded poly‐A loop. Also incorporated in the structure is a poly‐T trigger strand bearing seven photocaged residues. Upon illumination with 365 nm light, the cages are removed and the trigger strand hybridizes to the loop, opening the tweezer and increasing the distance between its arms from 4 to 18 nm. This intramolecular process is roughly 60 times faster than adding an external trigger strand, and provides a mechanism for the rapid interconversion of DNA nanostructures with light.