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Site‐Specific Labeling of Affimers for DNA‐PAINT Microscopy
Author(s) -
Schlichthaerle Thomas,
Eklund Alexandra S.,
Schueder Florian,
Strauss Maximilian T.,
Tiede Christian,
Curd Alistair,
Ries Jonas,
Peckham Michelle,
Tomlinson Darren C.,
Jungmann Ralf
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201804020
Subject(s) - aptamer , dna , fluorescence microscope , nanotechnology , microscopy , super resolution microscopy , superresolution , fluorescence , fluorescence lifetime imaging microscopy , conjugated system , resolution (logic) , chemistry , materials science , biophysics , computer science , biology , microbiology and biotechnology , image (mathematics) , optics , physics , artificial intelligence , biochemistry , polymer , organic chemistry
Optical super‐resolution techniques allow fluorescence imaging below the classical diffraction limit of light. From a technology standpoint, recent methods are approaching molecular‐scale spatial resolution. However, this remarkable achievement is not easily translated to imaging of cellular components, since current labeling approaches are limited by either large label sizes (antibodies) or the sparse availability of small and efficient binders (nanobodies, aptamers, genetically‐encoded tags). In this work, we combined recently developed Affimer reagents with site‐specific DNA modification for high‐efficiency labeling and imaging using DNA‐PAINT. We assayed our approach using an actin Affimer. The small DNA‐conjugated affinity binders could provide a solution for efficient multitarget super‐resolution imaging in the future.