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On the Stability of DNA Origami Nanostructures in Low‐Magnesium Buffers
Author(s) -
Kielar Charlotte,
Xin Yang,
Shen Boxuan,
Kostiainen Mauri A.,
Grundmeier Guido,
Linko Veikko,
Keller Adrian
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201802890
Subject(s) - dna origami , dna , dna nanotechnology , nanotechnology , nanostructure , superstructure , chemistry , materials science , biophysics , biochemistry , physics , biology , thermodynamics
DNA origami structures have great potential as functional platforms in various biomedical applications. Many applications, however, are incompatible with the high Mg 2+ concentrations commonly believed to be a prerequisite for maintaining DNA origami integrity. Herein, we investigate DNA origami stability in low‐Mg 2+ buffers. DNA origami stability is found to crucially depend on the availability of residual Mg 2+ ions for screening electrostatic repulsion. The presence of EDTA and phosphate ions may thus facilitate DNA origami denaturation by displacing Mg 2+ ions from the DNA backbone and reducing the strength of the Mg 2+ –DNA interaction, respectively. Most remarkably, these buffer dependencies are affected by DNA origami superstructure. However, by rationally selecting buffer components and considering superstructure‐dependent effects, the structural integrity of a given DNA origami nanostructure can be maintained in conventional buffers even at Mg 2+ concentrations in the low‐micromolar range.