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Valency‐Controlled Framework Nucleic Acid Signal Amplifiers
Author(s) -
Liu Qi,
Ge Zhilei,
Mao Xiuhai,
Zhou Guobao,
Zuo Xiaolei,
Shen Juwen,
Shi Jiye,
Li Jiang,
Wang Lihua,
Chen Xiaoqing,
Fan Chunhai
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201802701
Subject(s) - nucleic acid , biosensor , biomolecule , amplifier , signal (programming language) , nanotechnology , chemistry , biophysics , materials science , biology , computer science , biochemistry , optoelectronics , cmos , programming language
Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in in vitro biomimetic systems generally lack the spatiotemporal regulation in vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy to develop valence‐controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence‐controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor‐relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3–5 orders of magnitude and improved dynamic range.