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Cover Picture: Nucleophile Promiscuity of Engineered Class II Pyruvate Aldolase YfaU from E. Coli (Angew. Chem. Int. Ed. 14/2018)
Author(s) -
Hernández Karel,
Joglar Jesús,
Bujons Jordi,
Parella Teodor,
Clapés Pere
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201802275
Subject(s) - nucleophile , aldolase a , electrophile , chemistry , pyrrolizidine , residue (chemistry) , stereochemistry , organic chemistry , catalysis , enzyme
Structure‐guided protein engineering of the class II pyruvate aldolase YfaU from E. coli is described. In their Communication on page 3583 ff., P. Clapés and co‐workers report the resulting straightforward access to chiral building blocks and proline and pyrrolizidine α‐amino acid derivatives. The picture shows how YfaU works like a timing gear to bring together the nucleophile and electrophile to give C−C bond formation. The timing gear has approached the nucleophile at the reactive residue and activation of the nucleophile has occurred (light yellow).