Premium
Two Diterpene Synthases for Spiroalbatene and Cembrene A from Allokutzneria albata
Author(s) -
Rinkel Jan,
Lauterbach Lukas,
Rabe Patrick,
Dickschat Jeroen S.
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201800385
Subject(s) - diterpene , enzyme , stereochemistry , mutagenesis , cofactor , biochemistry , chemistry , atp synthase , biosynthesis , biology , gene , mutation
Two bacterial diterpene synthases from the actinomycete Allokutzneria albata were investigated, resulting in the identification of the structurally unprecedented compound spiroalbatene from the first and cembrene A from the second enzyme. Both enzymes were thoroughly investigated in terms of their mechanisms by isotope labeling experiments, site‐directed mutagenesis, and variation of the metal cofactors and pH value. For spiroalbatene synthase, the pH‐ and Mn 2+ ‐dependent formation of the side product thunbergol was observed, which is biosynthetically linked to spiroalbatene.