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Natural Product Diversification Mediated by Alternative Transcriptional Starting
Author(s) -
Tobias Nicholas J.,
Linck Annabell,
Bode Helge B.
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201713199
Subject(s) - biology , photorhabdus luminescens , photorhabdus , gene , natural product , computational biology , genetics , biochemistry
Photorhabdus luminescens dedicates a significant proportion of its genome to the production of natural products. These products and the structural variation in their derivatives may occur by a number of well‐described mechanisms, such as module skipping or precursor promiscuity. Cappable‐seq was used to identify transcriptional start sites of many of the gene clusters present in P. luminescens TTO1. We discovered that variations associated with the non‐ribosomal peptide synthetase Kol, which is responsible for kolossin A production, possessed a number of internal transcripts that lead to synthesis of the smaller kolossin derivatives kolossin B and C. The data here support a new mechanism of natural product biosynthetic variation whereby mRNA may code for shorter NRPS enzymes in addition to full‐length proteins, resulting in the production of smaller peptide derivatives.