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Rapid Analysis of Cell–Nanoparticle Interactions using Single‐Cell Raman Trapping Microscopy
Author(s) -
Steinke Maria,
Zunhammer Florian,
Chatzopoulou Elisavet I.,
Teller Henrik,
Schütze Karin,
Walles Heike,
Rädler Joachim O.,
Grüttner Cordula
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201713151
Subject(s) - raman spectroscopy , nanoparticle , particle (ecology) , nanotechnology , materials science , cytotoxicity , nanoparticle tracking analysis , microscopy , cell , fluorescence microscope , biophysics , chemistry , fluorescence , medicine , optics , pathology , biology , in vitro , biochemistry , physics , ecology , microrna , microvesicles , gene
Iron oxide nanoparticles have been used in preclinical studies to label stem cells for non‐invasive tracking and homing. The search continues for novel particle candidates that are suitable for clinical applications. Since standard analyses to investigate cell–particle interactions and safety are labor‐intensive, an efficient procedure is required to guide future particle development and to exclude adverse health effects. The application of combined Raman trapping microscopy with fluidic chips is reported for the analysis of single cells labeled with different types of aminated iron oxide particles. Multivariate data analysis revealed Raman signal differences that could be clearly assigned to cell–particle interactions and cytotoxicity, respectively. A validation dataset verified that more than 95 % of the spectra were correctly classified. Thus, our approach enables rapid discrimination of non‐hazardous from cytotoxic nanoparticles as a prerequisite for safe clinical applications.