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CO 2 to Terpenes: Autotrophic and Electroautotrophic α‐Humulene Production with Cupriavidus necator
Author(s) -
Krieg Thomas,
Sydow Anne,
Faust Sonja,
Huth Ina,
Holtmann Dirk
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201711302
Subject(s) - cupriavidus necator , humulene , terpene , chemistry , raw material , botany , organic chemistry , food science , bacteria , biology , essential oil , polyhydroxyalkanoates , genetics
We show that CO 2 can be converted by an engineered “Knallgas” bacterium ( Cupriavidus necator ) into the terpene α‐humulene. Heterologous expression of the mevalonate pathway and α‐humulene synthase resulted in the production of approximately 10 mg α‐humulene per gram cell dry mass (CDW) under heterotrophic conditions. This first example of chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen is a promising starting point for the production of different high‐value terpene compounds from abundant and simple raw materials. Furthermore, the production system was used to produce 17 mg α‐humulene per gram CDW from CO 2 and electrical energy in microbial electrosynthesis (MES) mode. Given that the system can convert CO 2 by using electrical energy from solar energy, it opens a new route to artificial photosynthetic systems.