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In Situ Scatheless Cell Detachment Reveals Correlation between Adhesion Strength and Viability at Single‐Cell Resolution
Author(s) -
Mao Sifeng,
Zhang Wanling,
Huang Qiushi,
Khan Mashooq,
Li Haifang,
Uchiyama Katsumi,
Lin JinMing
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201710273
Subject(s) - single cell analysis , cell , cell adhesion , microfluidics , in situ , adhesion , microbiology and biotechnology , resolution (logic) , biology , viability assay , nanotechnology , computational biology , computer science , chemistry , materials science , artificial intelligence , genetics , organic chemistry
Single‐cell biology provides insights into some of the most fundamental processes in biology and promotes the understanding of life's mysteries. As the technologies to study single‐cells expand, they will require sophisticated analytical tools to make sense of various behaviors and components of single‐cells as well as their relations in the adherent tissue culture. In this paper, we revealed cell heterogeneity and uncovered the connections between cell adhesion strength and cell viability at single‐cell resolution by extracting single adherent cells of interest from a standard tissue culture by using a microfluidic chip‐based live single‐cell extractor (LSCE). We believe that this method will provide a valuable new tool for single‐cell biology.