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Folding DNA into a Lipid‐Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins
Author(s) -
Dong Yuanchen,
Chen Shuobing,
Zhang Shijian,
Sodroski Joseph,
Yang Zhongqiang,
Liu Dongsheng,
Mao Youdong
Publication year - 2018
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201710147
Subject(s) - nanodisc , dna origami , membrane , membrane protein , dna , biophysics , chemistry , folding (dsp implementation) , integral membrane protein , dna nanotechnology , microbiology and biotechnology , nanotechnology , biochemistry , biology , materials science , electrical engineering , engineering
Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self‐assembled DNA nanobarrel that scaffolds a nanodisc‐like lipid environment. Compared with the membrane‐scaffolding‐protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.