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Modulation of Quorum Sensing in a Gram‐Positive Pathogen by Linear Molecularly Imprinted Polymers with Anti‐infective Properties
Author(s) -
Motib Anfal,
Guerreiro Antonio,
AlBayati Firas,
Piletska Elena,
Manzoor Irfan,
Shafeeq Sulman,
Kadam Anagha,
Kuipers Oscar,
Hiller Luisa,
Cowen Todd,
Piletsky Sergey,
Andrew Peter W.,
Yesilkaya Hasan
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201709313
Subject(s) - quorum sensing , molecularly imprinted polymer , linker , monomer , chemistry , combinatorial chemistry , molecular imprinting , in vivo , virulence , polymer , biochemistry , biology , selectivity , organic chemistry , gene , microbiology and biotechnology , computer science , operating system , catalysis
We describe the development, characterization, and biological testing of a new type of linear molecularly imprinted polymer (LMIP) designed to act as an anti‐infective by blocking the quorum sensing (QS) mechanism and so abrogating the virulence of the pathogen Streptococcus pneumoniae . The LMIP is prepared (polymerized) in presence of a template molecule, but unlike in traditional molecular imprinting approaches, no cross‐linker is used. This results in soluble low‐molecular‐weight oligomers that can act as a therapeutic agent in vitro and in vivo. The LMIP was characterized by mass spectrometry to determine its monomer composition. Fragments identified were then aligned along the peptide template by computer modeling to predict the possible monomer sequence of the LMIP. These findings provide a proof of principle that LMIPs can be used to block QS, thus setting the stage for the development of LMIPs a novel drug‐discovery platform and class of materials to target Gram‐positive pathogens.