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Single‐Molecule Observation of the Photoregulated Conformational Dynamics of DNA Origami Nanoscissors
Author(s) -
Willner Elena M.,
Kamada Yuu,
Suzuki Yuki,
Emura Tomoko,
Hidaka Kumi,
Dietz Hendrik,
Sugiyama Hiroshi,
Endo Masayuki
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201708722
Subject(s) - atomic force microscopy , conformational change , dna origami , chemistry , irradiation , ultraviolet light , visible spectrum , molecule , biophysics , dna , ultraviolet , crystallography , photochemistry , nanotechnology , materials science , stereochemistry , optoelectronics , biochemistry , physics , organic chemistry , nuclear physics , biology
Abstract We demonstrate direct observation of the dynamic opening and closing behavior of photocontrollable DNA origami nanoscissors using high‐speed atomic force microscopy (HS‐AFM). First the conformational change between the open and closed state controlled by adjustment of surrounding salt concentration could be directly observed during AFM scanning. Then light‐responsive moieties were incorporated into the nanoscissors to control these structural changes by photoirradiation. Using photoswitchable DNA strands, we created a photoresponsive nanoscissors variant and were able to distinguish between the open and closed conformations after respective irradiation with ultraviolet (UV) and visible (Vis) light by gel electrophoresis and AFM imaging. Additionally, these reversible changes in shape during photoirradiation were directly visualized using HS‐AFM. Moreover, four photoswitchable nanoscissors were assembled into a scissor–actuator‐like higher‐order object, the configuration of which could be controlled by the open and closed switching induced by irradiation with UV and Vis light.