Premium
Luciferases with Tunable Emission Wavelengths
Author(s) -
Hiblot Julien,
Yu Qiuliyang,
Sabbadini Marina D.B.,
Reymond Luc,
Xue Lin,
Schena Alberto,
Sallin Olivier,
Hill Nicholas,
Griss Rudolf,
Johnsson Kai
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201708277
Subject(s) - luciferases , fluorophore , wavelength , chemistry , fluorescence , photochemistry , optoelectronics , materials science , physics , optics , luciferase , biochemistry , gene , transfection
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP‐tag or HaloTag7. Labeling of the self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.