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Because the Light is Better Here: Correlation‐Time Analysis by NMR Spectroscopy
Author(s) -
Smith Albert A.,
Ernst Matthias,
Meier Beat H.
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201707316
Subject(s) - relaxation (psychology) , correlation function (quantum field theory) , statistical physics , dynamics (music) , exponential function , correlation , motion (physics) , function (biology) , physics , nuclear magnetic resonance , mathematics , mathematical analysis , classical mechanics , statistics , spectral density , geometry , psychology , social psychology , evolutionary biology , acoustics , biology
Relaxation data in NMR spectra are often used for dynamics analysis, by modeling motion in the sample with a correlation function consisting of one or more decaying exponential terms, each described by an order parameter, and a correlation time. This method has its origins in the Lipari–Szabo model‐free approach, which originally considered overall tumbling plus one internal motion and was later expanded to several internal motions. Considering several of these cases in the solid state it is found that if the real motion is more complex than the assumed model, model fitting is biased towards correlation times where the relaxation data are most sensitive. This leads to unexpected distortions in the resulting dynamics description. Therefore dynamics detectors should be used, which characterize different ranges of correlation times and can help in the analysis of protein motion without assuming a specific model of the correlation function.