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Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers
Author(s) -
Graham Ben,
Bailey Trisha L.,
Healey Joseph R. J.,
Marcellini Moreno,
Deville Sylvain,
Gibson Matthew I.
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201706703
Subject(s) - polyproline helix , cryoprotectant , cryopreservation , antifreeze protein , chemistry , dimethyl sulfoxide , antifreeze , biophysics , biochemistry , microbiology and biotechnology , biology , peptide , organic chemistry , embryo
Tissue engineering, gene therapy, drug screening, and emerging regenerative medicine therapies are fundamentally reliant on high‐quality adherent cell culture, but current methods to cryopreserve cells in this format can give low cell yields and require large volumes of solvent “antifreezes”. Herein, we report polyproline as a minimum (bio)synthetic mimic of antifreeze proteins that is accessible by solution, solid‐phase, and recombinant methods. We demonstrate that polyproline has ice recrystallisation inhibition activity linked to its amphipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines. Polyproline may be a versatile additive in the emerging field of macromolecular cryoprotectants.