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Laser‐Initiated Radical Trifluoromethylation of Peptides and Proteins: Application to Mass‐Spectrometry‐Based Protein Footprinting
Author(s) -
Cheng Ming,
Zhang Bojie,
Cui Weidong,
Gross Michael L.
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201706697
Subject(s) - trifluoromethylation , footprinting , chemistry , mass spectrometry , myoglobin , dna footprinting , combinatorial chemistry , reagent , biochemistry , organic chemistry , chromatography , dna binding protein , dna , alkyl , trifluoromethyl , transcription factor , base sequence , gene
Described is a novel, laser‐initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. . CF 3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to . OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.