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Recognition‐then‐Reaction Enables Site‐Selective Bioconjugation to Proteins on Live‐Cell Surfaces
Author(s) -
Cui Cheng,
Zhang Hui,
Wang Ruowen,
Cansiz Sena,
Pan Xiaoshu,
Wan Shuo,
Hou Weijia,
Li Long,
Chen Meiwan,
Liu Yuan,
Chen Xigao,
Liu Qiaoling,
Tan Weihong
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201706285
Subject(s) - aptamer , bioconjugation , selectivity , chemistry , target protein , combinatorial chemistry , surface modification , dna , molecular recognition , avidin , biochemistry , nanotechnology , microbiology and biotechnology , biology , biotin , gene , materials science , organic chemistry , molecule , catalysis
Site‐selective protein modification is a key step in facilitating protein functionalization and manipulation. To accomplish this, genetically engineered proteins were previously required, but the procedure was laborious, complex, and technically challenging. Herein we report the development of aptamer‐based recognition‐then‐reaction to guide site‐selective protein/DNA conjugation in a single step with outstanding selectivity and efficiency. As models, several proteins, including human thrombin, PDGF‐BB, Avidin, and His‐tagged recombinant protein, were studied, and the results showed excellent selectivity under mild reaction conditions. Taking advantage of aptamers as recognition elements with extraordinary selectivity and affinity, this simple preparation method can tag a protein in a complex milieu. Thus, with the aptamer obtained from cell‐SELEX, real‐time modification of live‐cell membrane proteins can be achieved in one step without any pre‐treatment.