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HKOH‐1: A Highly Sensitive and Selective Fluorescent Probe for Detecting Endogenous Hydroxyl Radicals in Living Cells
Author(s) -
Bai Xiaoyu,
Huang Yueyang,
Lu Mingyang,
Yang Dan
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201705873
Subject(s) - endogeny , radical , reactive oxygen species , hydroxyl radical , fluorescence , chemistry , flow cytometry , biophysics , photochemistry , chemiluminescence , hela , confocal , reactivity (psychology) , in vitro , biochemistry , biology , microbiology and biotechnology , chromatography , medicine , physics , geometry , mathematics , alternative medicine , quantum mechanics , pathology
The hydroxyl radical ( . OH), one of the most reactive and deleterious reactive oxygen species (ROS), has been suggested to play an essential role in many physiological and pathological scenarios. However, a reliable and robust method to detect endogenous . OH is currently lacking owing to its extremely high reactivity and short lifetime. Herein we report a fluorescent probe HKOH‐1 with superior in vitro selectivity and sensitivity towards . OH. With this probe, we have calibrated and quantified the scavenging capacities of a wide range of reported . OH scavengers. Furthermore, HKOH‐1r, which was designed for better cellular uptake and retention, has performed robustly in detection of endogenous . OH generation by both confocal imaging and flow cytometry. Furthermore, this probe has been applied to monitor . OH generation in HeLa cells in response to UV light irradiation. Therefore, HKOH‐1 could be used for elucidating . OH related biological functions.