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Dendronized Semiconducting Polymer as Photothermal Nanocarrier for Remote Activation of Gene Expression
Author(s) -
Lyu Yan,
Cui Dong,
Sun He,
Miao Yansong,
Duan Hongwei,
Pu Kanyi
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201705543
Subject(s) - photothermal therapy , transgene , nanocarriers , transduction (biophysics) , photothermal effect , gene expression , intracellular , transcription (linguistics) , microbiology and biotechnology , nanotechnology , gene , chemistry , materials science , biophysics , biology , biochemistry , drug delivery , linguistics , philosophy
Regulation of transgene systems is needed to develop innovative medicines. However, noninvasive remote control of gene expression has been rarely developed and remains challenging. We herein synthesize a near‐infrared (NIR) absorbing dendronized semiconducting polymer (DSP) and utilize it as a photothermal nanocarrier not only to efficiently deliver genes but also to spatiotemporally control gene expression in conjunction with heat‐inducible promoter. DSP has a high photothermal conversion efficiency (44.2 %) at 808 nm, permitting fast transduction of NIR light into thermal signals for intracellular activation of transcription. Such a DSP‐mediated remote activation can rapidly and safely result in 25‐ and 4.5‐fold increases in the expression levels of proteins in living cells and mice, respectively. This study thus provides a promising approach to optically regulate transgene systems for on‐demand therapeutic transgene dosing.