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Ion‐Tagged Oligonucleotides Coupled with a Magnetic Liquid Support for the Sequence‐Specific Capture of DNA
Author(s) -
Clark Kevin D.,
Varona Marcelino,
Anderson Jared L.
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201703299
Subject(s) - oligonucleotide , nucleic acid , dna , magnetic nanoparticles , chemistry , combinatorial chemistry , nucleotide , nanotechnology , materials science , nanoparticle , biochemistry , gene
The isolation of specific nucleic acid sequences is a major bottleneck in molecular diagnostics. Magnetic beads/particles are typically used as solid supports for the capture of DNA targets to improve sample throughput but aggregate over time resulting in lower capture efficiency and obstruction of liquid handling devices. Herein, we describe a particle‐free approach to sequence‐specific DNA extraction using a magnetic liquid support and ion‐tagged oligonucleotide (ITO) probes. ITO conjugates were synthesized with the highest yields ever achieved for the radical thiol‐ene coupling of a substrate and oligonucleotide. In addition to distinguishing nucleotide mismatches, the ITO and magnetic liquid‐based approach was more sensitive than a commercial magnetic bead‐based method for the capture of target DNA from a pool of interfering genomic DNA.