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Utilizing Paper‐Based Devices for Antimicrobial‐Resistant Bacteria Detection
Author(s) -
Boehle Katherine E.,
Gilliand Jake,
Wheeldon Christopher R.,
Holder Amethyst,
Adkins Jaclyn A.,
Geiss Brian J.,
Ryan Elizabeth P.,
Henry Charles S.
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201702776
Subject(s) - antimicrobial , antibiotic resistance , bacteria , antimicrobial drug , contamination , wastewater , drug resistance , sewage , microbiology and biotechnology , biology , environmental science , environmental engineering , ecology , genetics
Abstract Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents. Per the World Health Organization, AMR has an estimated annual cost of USD 34 billion in the US and is predicted to be the number one cause of death worldwide by 2050. One way AMR bacteria can spread, and by which individuals can contract AMR infections, is through contaminated water. Monitoring AMR bacteria in the environment currently requires that samples be transported to a central laboratory for slow and labor intensive tests. We have developed an inexpensive assay using paper‐based analytical devices (PADs) that can test for the presence of β‐lactamase‐mediated resistance. To demonstrate viability, the PAD was used to detect β‐lactam resistance in wastewater and sewage and identified resistance in individual bacterial species isolated from environmental water sources.