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A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping
Author(s) -
Yuyama Kenichi,
Ueda Mariko,
Nagao Satoshi,
Hirota Shun,
Sugiyama Teruki,
Masuhara Hiroshi
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201702352
Subject(s) - amyloid (mycology) , fibril , nucleation , microstructure , materials science , biophysics , amyloid fibril , nanotechnology , crystallography , chemistry , amyloid β , biology , medicine , inorganic chemistry , disease , organic chemistry , pathology
Protein amyloids have received much attention owing to their correlation with serious diseases and to their promising mechanical and optical properties as future materials. Amyloid formation has been conducted by tuning temperature and chemical conditions, so that its nucleation and the following growth are analyzed as ensemble dynamics. A single spherical assembly of amyloid fibrils of cytochrome c domain‐swapped dimer was successfully generated upon laser trapping. The amyloid fibrillar structure was confirmed by fluorescence characterization and electron microscopy. The prepared spheres were further manipulated individually in solution to fabricate a three‐dimensional microstructure and a line pattern. Amyloid formation dynamics and amyloid‐based microstructure fabrication are demonstrated based on direct observation of a single spherical assembly, which foresees a new approach in amyloid studies.