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Specific Identification of Glycoproteins Bearing the Tn Antigen in Human Cells
Author(s) -
Zheng Jiangnan,
Xiao Haopeng,
Wu Ronghu
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201702191
Subject(s) - glycoprotein , glycan , antigen , glycopeptide , chemistry , biochemistry , jurkat cells , microbiology and biotechnology , computational biology , biology , immunology , t cell , immune system , antibiotics
Abstract Glycoproteins contain a wealth of valuable information regarding the development and disease status of cells. In cancer cells, some glycans (such as the Tn antigen) are highly up‐regulated, but this remains largely unknown for glycoproteins with a particular glycan. Herein, an innovative method combining enzymatic and chemical reactions was first designed to enrich glycoproteins with the Tn antigen. Using synthetic glycopeptides with O‐GalNAc (the Tn antigen) or O‐GlcNAc, we demonstrated that the method is selective for glycopeptides with O‐GalNAc and can distinguish between these two modifications. The diagnostic ions from the tagged O‐GalNAc further confirmed the effectiveness of the method and confidence in the identification of glycopeptides with the Tn antigen by mass spectrometry. Using this method, we identified 96 glycoproteins with the Tn antigen in Jurkat cells. The method can be extensively applied in biological and biomedical research.