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A Reversible Fluorescent Probe for Real‐Time Quantitative Monitoring of Cellular Glutathione
Author(s) -
Liu Zhixue,
Zhou Xin,
Miao Yu,
Hu Ying,
Kwon Nahyun,
Wu Xue,
Yoon Juyoung
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201702114
Subject(s) - glutathione , fluorescence , hela , chemistry , biophysics , dissociation constant , dissociation (chemistry) , cytotoxicity , biochemistry , in vitro , biology , enzyme , optics , physics , receptor
The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH‐related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG‐ 1 ) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant ( K d ) of 2.59 m m and a fast response time ( t 1/2 =5.82 s). We also demonstrate that QG‐ 1 detection of GSH is feasible in a model protein system. QG‐ 1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 m m ).