Premium
Synthetic Glycoforms Reveal Carbohydrate‐Dependent Bioactivity of Human Saposin D
Author(s) -
Graf Christopher G. F.,
Schulz Christian,
Schmälzlein Marina,
Heinlein Christian,
Mönnich Manuel,
Perkams Lukas,
Püttner Markus,
Boos Irene,
Hessefort Markus,
Lombana Sanchez Jose Nelson,
Weyand Michael,
Steegborn Clemens,
Breiden Bernadette,
Ross Kerstin,
Schwarzmann Günter,
Sandhoff Konrad,
Unverzagt Carlo
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201701362
Subject(s) - glycan , biochemistry , glycoprotein , carbohydrate , moiety , chemistry , biology , stereochemistry
The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid‐phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid‐binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N‐glycan.