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Dynamic Modulation of Enzyme Activity by Near‐Infrared Light
Author(s) -
Wang Changping,
Zhang Qiang,
Wang Xinyu,
Chang Hong,
Zhang Sanjun,
Tang Yuankai,
Xu Jianhua,
Qi Ruijuan,
Cheng Yiyun
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201700968
Subject(s) - enzyme , irradiation , photothermal effect , near infrared spectroscopy , catalase , chemistry , photothermal therapy , materials science , nanotechnology , biochemistry , optics , physics , nuclear physics
Engineering near‐infrared (NIR) light‐sensitive enzymes remains a huge challenge. A photothermal effect‐associated method is developed for tailoring the enzymatic activity of enzymes by exposure to NIR light. An ultrasmall platinum nanoparticle was anchored in an enzyme to generate local heating upon NIR irradiation, which enhanced the enzyme activity without increasing bulk temperature. Following NIR irradiation, the enzyme activity was tailored rapidly and reversibly, and was modulated by varying laser power density and irradiation time. Four enzymes were engineered, including glucoamylase, glucose oxidase, catalase, and proteinase K with NIR‐light sensitivity, and demonstrated their utility in practical applications such as photolithography and NIR light‐responsive antibacterial or anticancer actions. Our investigation suggests that this approach could be broadly used to engineer enzymes with NIR‐light sensitivity for many biological applications.