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Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi
Author(s) -
Yang Yanlong,
Zhang Shasha,
Ma Ke,
Xu Yuxing,
Tao Qiaoqiao,
Chen Yihua,
Chen Juan,
Guo Shunxing,
Ren Jinwei,
Wang Wenzhao,
Tao Yong,
Yin WenBing,
Liu Hongwei
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201700565
Subject(s) - diterpene , bacteria , biology , escherichia coli , biochemistry , enzyme , gene , genetics
Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum . Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA‐related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily.