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Green‐Light‐Induced Inactivation of Receptor Signaling Using Cobalamin‐Binding Domains
Author(s) -
Kainrath Stephanie,
Stadler Manuela,
Reichhart Eva,
Distel Martin,
Janovjak Harald
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201611998
Subject(s) - microbiology and biotechnology , optogenetics , receptor , zebrafish , cobalamin , fibroblast growth factor , biology , signal transduction , transcription factor , fibroblast growth factor receptor , biophysics , chemistry , biochemistry , neuroscience , vitamin b12 , gene
Optogenetics and photopharmacology provide spatiotemporally precise control over protein interactions and protein function in cells and animals. Optogenetic methods that are sensitive to green light and can be used to break protein complexes are not broadly available but would enable multichromatic experiments with previously inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12) binding domains of bacterial CarH transcription factors for green‐light‐induced receptor dissociation. In cultured cells, we observed oligomerization‐induced cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin‐binding domains in the dark that was rapidly eliminated upon illumination. In zebrafish embryos expressing fusion receptors, green light endowed control over aberrant fibroblast growth factor signaling during development. Green‐light‐induced domain dissociation and light‐inactivated receptors will critically expand the optogenetic toolbox for control of biological processes.