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Universal Super‐Resolution Multiplexing by DNA Exchange
Author(s) -
Schueder Florian,
Strauss Maximilian T.,
Hoerl David,
Schnitzbauer Joerg,
Schlichthaerle Thomas,
Strauss Sebastian,
Yin Peng,
Harz Hartmann,
Leonhardt Heinrich,
Jungmann Ralf
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201611729
Subject(s) - multiplexing , sted microscopy , computer science , resolution (logic) , image resolution , limit (mathematics) , dna , superresolution , nanotechnology , physics , artificial intelligence , image (mathematics) , biology , materials science , telecommunications , mathematics , genetics , mathematical analysis
Abstract Super‐resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20× higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time‐consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super‐resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.