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Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)
Author(s) -
Nikić Ivana,
Estrada Girona Gemma,
Kang Jun Hee,
Paci Giulia,
Mikhaleva Sofya,
Koehler Christine,
Shymanska Nataliia V.,
Ventura Santos Camilla,
Spitz Daniel,
Lemke Edward A.
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201611395
Subject(s) - click chemistry , amino acid , genetic code , dna , biophysics , chemistry , biochemistry , materials science , biology , combinatorial chemistry
Cytoplasmic re‐targeting of the pyrrolysine‐based genetic code expansion machinery by using a nuclear export signal (NES) substantially increases the site‐specific incorporation of clickable amino acids (green) into proteins in mammalian cells, as shown by E. A. Lemke and co‐workers in their Communication on page 16172 ff. These clickable amino acids can then be modified with annealing sites for small DNA imaging strands (red) to enable high‐contrast Click‐PAINT super‐resolution microscopy.