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Real‐Time Monitoring of Nanoparticle Formation by FRET Imaging
Author(s) -
SanchezGaytan Brenda L.,
Fay François,
Hak Sjoerd,
Alaarg Amr,
Fayad Zahi A.,
PérezMedina Carlos,
Mulder Willem J. M.,
Zhao Yiming
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201611288
Subject(s) - förster resonance energy transfer , nanoparticle , nanotechnology , micelle , microfluidics , nanostructure , nanocrystal , materials science , chemistry , fluorescence , organic chemistry , physics , quantum mechanics , aqueous solution
Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self‐assembled nanoparticles whose formation processes have been largely overlooked. Herein, we present a new technology that integrates a microfluidic‐based nanoparticle synthesis method and Förster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self‐assembly in real time. Applied to different nanoparticle systems, for example, nanoemulsions, drug‐loaded block‐copolymer micelles, and nanocrystal‐core reconstituted high‐density lipoproteins, we have shown the approach's unique ability to investigate key parameters affecting nanoparticle formation.