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GXXXG‐Mediated Parallel and Antiparallel Dimerization of Transmembrane Helices and Its Inhibition by Cholesterol: Single‐Pair FRET and 2D IR Studies
Author(s) -
Yano Yoshiaki,
Kondo Kotaro,
Watanabe Yuta,
Zhang Tianqi O.,
Ho JiaJung,
Oishi Shinya,
Fujii Nobutaka,
Zanni Martin T.,
Matsuzaki Katsumi
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201609708
Subject(s) - antiparallel (mathematics) , förster resonance energy transfer , liposome , transmembrane domain , helix (gastropod) , biophysics , crystallography , chemistry , structural motif , transmembrane protein , membrane , biology , biochemistry , physics , fluorescence , ecology , receptor , quantum mechanics , snail , magnetic field
Small‐residue‐mediated interhelical packings are ubiquitously found in helical membrane proteins, although their interaction dynamics and lipid dependence remain mostly uncharacterized. We used a single‐pair FRET technique to examine the effect of a GXXXG motif on the association of de novo designed (AALALAA) 3 helices in liposomes. Dimerization occurred with sub‐second lifetimes, which was abolished by cholesterol. Utilizing the nearly instantaneous time‐resolution of 2D IR spectroscopy, parallel and antiparallel helix associations were identified by vibrational couplings across helices at their interface. Taken together, the data illustrate that the GXXXG motif controls helix packing but still allows for a dynamic and lipid‐regulated oligomeric state.