Premium
Multilayer Microcapsules for FRET Analysis and Two‐Photon‐Activated Photodynamic Therapy
Author(s) -
Yang Yang,
Liu Huiling,
Han Mingjuan,
Sun Bingbing,
Li Junbai
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201605905
Subject(s) - förster resonance energy transfer , two photon excitation microscopy , fluorescence , photodynamic therapy , microscopy , fluorescence lifetime imaging microscopy , materials science , confocal microscopy , fluorescence in the life sciences , confocal , laser , fluorescence microscope , spectroscopy , photon , fluorescence spectroscopy , optoelectronics , nanotechnology , chemistry , optics , physics , organic chemistry , quantum mechanics
Microcapsules obtained by layer‐by‐layer assembly provide a good platform for biological analysis owing to their component diversity, multiple binding sites, and controllable wall thickness. Herein, different assembly species were obtained from two‐photon dyes and traditional photosensitizers, and further assembled into microcapsules. Fluorescence resonance energy transfer (FRET) was shown to occur between the two‐photon dyes and photosensitizers. Confocal laser scanning microscopy (CLSM) with one‐ and two‐photon lasers, fluorescence lifetime imaging microscopy (FLIM), and time‐resolved fluorescence spectroscopy were used to analyze the FRET effects in the microcapsules. The FRET efficiency could easily be controlled through changing the assembly sequence. Furthermore, the capsules are phototoxic upon one‐ or two‐photon excitation. These materials are thus expected to be applicable in two‐photon‐activated photodynamic therapy for deep‐tissue treatment.