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Designer Extracellular Matrix Based on DNA–Peptide Networks Generated by Polymerase Chain Reaction
Author(s) -
Finke Alexander,
Bußkamp Holger,
Manea Marilena,
Marx Andreas
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201604687
Subject(s) - extracellular matrix , peptide , dna , cell adhesion , covalent bond , chemistry , integrin , extracellular , microbiology and biotechnology , cell , multicellular organism , adhesion , matrix (chemical analysis) , biochemistry , biophysics , biology , organic chemistry , chromatography
Cell proliferation and differentiation in multicellular organisms are partially regulated by signaling from the extracellular matrix. The ability to mimic an extracellular matrix would allow particular cell types to be specifically recognized, which is central to tissue engineering. We present a new functional DNA‐based material with cell‐adhesion properties. It is generated by using covalently branched DNA as primers in PCR. These primers were functionalized by click chemistry with the cyclic peptide c(RGDfK), a peptide that is known to predominantly bind to αvβ3 integrins, which are found on endothelial cells and fibroblasts, for example. As a covalent coating of surfaces, this DNA‐based material shows cell‐repellent properties in its unfunctionalized state and gains adhesiveness towards specific target cells when functionalized with c(RGDfK). These cells remain viable and can be released under mild conditions by DNase I treatment.