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Three‐Dimensional Control of DNA Hybridization by Orthogonal Two‐Color Two‐Photon Uncaging
Author(s) -
Fichte Manuela A. H.,
Weyel Xenia M. M.,
Junek Stephan,
Schäfer Florian,
Herbivo Cyril,
Goeldner Maurice,
Specht Alexandre,
Wachtveitl Josef,
Heckel Alexander
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201603281
Subject(s) - fluorescence , chemistry , two photon excitation microscopy , dna , photon , biophysics , photochemistry , optics , physics , biology , biochemistry
We successfully introduced two‐photon‐sensitive photolabile groups ([7‐(diethylamino)coumarin‐4‐yl]methyl and p ‐dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three‐dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double‐strand displacement in a hydrogel and in neurons. Orthogonal two‐photon uncaging of the two cages is possible, thus enabling complex scenarios of three‐dimensional control of hybridization with light.